› 1u1w

X-ray diffraction
1.35Å resolution

Structure and function of phenazine-biosynthesis protein PhzF from Pseudomonas fluorescens 2-79

Released:
DOI: 10.2210/pdb1u1w/pdb
PDB entry 1u1w coloured by chain, front view.
PDB entry 1u1w coloured by chain, side view.
PDB entry 1u1w coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens.
Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV
Proc Natl Acad Sci U S A 101 16431-6 (2004)
PMID: 15545603

Function and Biology Details

Reaction catalysed: 
(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxyate = (1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trans-2,3-dihydro-3-hydroxyanthranilate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 32.26 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51792 (Residues: 1-278; Coverage: 100%)
Gene name: phzF
Sequence domains: Phenazine biosynthesis-like protein
Structure domains: Diaminopimelate Epimerase; Chain A, domain 1

Ligands and Environments

3 bound ligands:
Ligand ACT 2 x ACT
Ligand 3HA 2 x 3HA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 92.6Å b: 99.86Å c: 57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.118 0.15
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Genomics of secondary metabolite production by Pseudomonas spp.
Gross et al. (2009)
10 more

4 mentions without citation

The structural biology of phenazine biosynthesis.
Blankenfeldt et al. (2014)
3 more