› 1u27

X-ray diffraction
2.3Å resolution

Triglycine variant of the ARNO Pleckstrin Homology Domain in complex with Ins(1,3,4,5)P4

Released:
DOI: 10.2210/pdb1u27/pdb
PDB entry 1u27 coloured by chain, front view.
PDB entry 1u27 coloured by chain, side view.
PDB entry 1u27 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural determinants of phosphoinositide selectivity in splice variants of Grp1 family PH domains.
Cronin TC, DiNitto JP, Czech MP, Lambright DG
EMBO J 23 3711-20 (2004)
PMID: 15359279

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytohesin-2 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 15.05 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63034 (Residues: 260-378; Coverage: 30%)
Gene names: Cyth2, Pscd2, Sec7b
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand 4IP 1 x 4IP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 41.917Å b: 56.024Å c: 59.533Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
14 more

13 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
12 more