› 1u29

X-ray diffraction
1.8Å resolution

Triglycine variant of the ARNO Pleckstrin Homology Domain in complex with Ins(1,4,5)P3

Released:
DOI: 10.2210/pdb1u29/pdb
Model geometry
Fit model/data
PDB entry 1u29 coloured by chain, front view.
PDB entry 1u29 coloured by chain, side view.
PDB entry 1u29 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural determinants of phosphoinositide selectivity in splice variants of Grp1 family PH domains.
Cronin TC, DiNitto JP, Czech MP, Lambright DG
EMBO J 23 3711-20 (2004)
PMID: 15359279

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytohesin-2 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 15.05 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63034 (Residues: 260-378; Coverage: 30%)
Gene names: Cyth2, Pscd2, Sec7b
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand I3P 1 x I3P
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
Spacegroup: P212121
Unit cell:
a: 42.998Å b: 56.378Å c: 57.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
14 more

9 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
8 more