› 1u2x

X-ray diffraction
2Å resolution

Crystal Structure of a Hypothetical ADP-dependent Phosphofructokinase from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb1u2x/pdb
PDB entry 1u2x coloured by chain, front view.
PDB entry 1u2x coloured by chain, side view.
PDB entry 1u2x coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
ADP-dependent 6-phosphofructokinase from Pyrococcus horikoshii OT3: structure determination and biochemical characterization of PH1645.
Currie MA, Merino F, Skarina T, Wong AH, Singer A, Brown G, Savchenko A, Caniuguir A, Guixé V, Yakunin AF, Jia Z
J Biol Chem 284 22664-71 (2009)
PMID: 19553681

Function and Biology Details

Reaction catalysed: 
ADP + D-fructose 6-phosphate = AMP + D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129851 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-specific phosphofructokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 474 amino acids
Theoretical weight: 54.82 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O59355 (Residues: 1-450; Coverage: 100%)
Gene names: PH1645, pfkC
Sequence domains: ADP-specific Phosphofructokinase/Glucokinase conserved region
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 68.065Å b: 99.932Å c: 82.581Å
α: 90° β: 110.38° γ: 90°
R-values:
R R work R free
0.199 0.174 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
8 other articles

4 mentions without citation

Ensemble approach to predict specificity determinants: benchmarking and validation.
Chakrabarti et al. (2009)
3 more