› 1u4c

X-ray diffraction
2.35Å resolution

Structure of spindle checkpoint protein Bub3

Released:
DOI: 10.2210/pdb1u4c/pdb
PDB entry 1u4c coloured by chain, front view.
PDB entry 1u4c coloured by chain, side view.
PDB entry 1u4c coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the spindle assembly checkpoint protein Bub3.
Larsen NA, Harrison SC
J Mol Biol 344 885-92 (2004)
PMID: 15544799

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150752 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell cycle arrest protein BUB3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 39.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P26449 (Residues: 1-341; Coverage: 100%)
Gene names: BUB3, OR26.16, YOR026W
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P32
Unit cell:
a: 73.266Å b: 73.266Å c: 110.425Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.196 0.288
Expression system: Escherichia coli

Quick links

Citations

4 review citations

On the road to cancer: aneuploidy and the mitotic checkpoint.
Kops et al. (2005)
3 more

4 mentions without citation

BUB1 and BUBR1: multifaceted kinases of the cell cycle.
Bolanos-Garcia et al. (2011)
3 more