› 1u6r

X-ray diffraction
1.65Å resolution

Transition state analog complex of muscle creatine kinase (R134K) mutant

Released:
DOI: 10.2210/pdb1u6r/pdb
PDB entry 1u6r coloured by chain, front view.
PDB entry 1u6r coloured by chain, side view.
PDB entry 1u6r coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structural asymmetry and intersubunit communication in muscle creatine kinase.
Ohren JF, Kundracik ML, Borders CL, Edmiston P, Viola RE
Acta Crystallogr D Biol Crystallogr 63 381-9 (2007)
PMID: 17327675

Function and Biology Details

Reaction catalysed: 
ATP + creatine = ADP + phosphocreatine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132720 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Creatine kinase M-type Chains: A, B
Molecule details ›
Chains: A, B
Length: 380 amino acids
Theoretical weight: 43.02 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00563 (Residues: 2-381; Coverage: 100%)
Gene name: CKM
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
Ligand NO3 1 x NO3
Ligand IOM 1 x IOM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 48.689Å b: 92.572Å c: 165.553Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.2
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Structural studies of human brain-type creatine kinase complexed with the ADP-Mg2+-NO3- -creatine transition-state analogue complex.
Bong et al. (2008)
10 more

12 mentions without citation

Cross-link guided molecular modeling with ROSETTA.
Kahraman et al. (2013)
11 more