› 1u78

X-ray diffraction
2.69Å resolution

Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA

Released:
DOI: 10.2210/pdb1u78/pdb
PDB entry 1u78 coloured by chain, front view.
PDB entry 1u78 coloured by chain, side view.
PDB entry 1u78 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structural analysis of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA.
Watkins S, van Pouderoyen G, Sixma TK
Nucleic Acids Res 32 4306-12 (2004)
PMID: 15304566

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114688 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposable element Tc3 transposase Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 16.21 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P34257 (Residues: 1-135; Coverage: 41%)
Gene names: B0303.5, tc3a
Sequence domains:
Structure domains:
26-MER Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 7.95 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided
26-MER Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 8.03 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P6122
Unit cell:
a: 93.681Å b: 93.681Å c: 255.558Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.233 0.273
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Integrating prokaryotes and eukaryotes: DNA transposases in light of structure.
Hickman et al. (2010)
5 more

8 mentions without citation

DDE transposases: Structural similarity and diversity.
Nesmelova et al. (2010)
7 more