› 1u92

X-ray diffraction
2.24Å resolution

Crystal structure of the HIV-1 Cross Neutralizing Monoclonal Antibody 2F5 in complex with gp41 Peptide Analog E-[Dap]-DKWQS (cyclic)

Released:
DOI: 10.2210/pdb1u92/pdb
PDB entry 1u92 coloured by chain, front view.
PDB entry 1u92 coloured by chain, side view.
PDB entry 1u92 coloured by chain, top view.
Source organisms:
Primary publication:
Crystallographic definition of the epitope promiscuity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2F5: vaccine design implications.
Bryson S, Julien JP, Hynes RC, Pai EF
J Virol 83 11862-75 (2009)
PMID: 19740978

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211585 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTIBODY 2F5 (LIGHT CHAIN) Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
ANTIBODY 2F5 (HEAVY CHAIN) Chain: B
Molecule details ›
Chain: B
Length: 234 amino acids
Theoretical weight: 24.85 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
GP41 PEPTIDE ANALOG Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 864 Da
Source organism: Human immunodeficiency virus 1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 58.1Å b: 65Å c: 175.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.249
Expression system: Not provided

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Citations

8 review citations

Broadly neutralizing antibodies present new prospects to counter highly antigenically diverse viruses.
Burton et al. (2012)
7 more

3 mentions without citation

Crystallographic definition of the epitope promiscuity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2F5: vaccine design implications.
Bryson et al. (2009)
2 more