› 1u9l

X-ray diffraction
1.9Å resolution

Structural basis for a NusA- protein N interaction

Released:
DOI: 10.2210/pdb1u9l/pdb
PDB entry 1u9l coloured by chain, front view.
PDB entry 1u9l coloured by chain, side view.
PDB entry 1u9l coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for the interaction of Escherichia coli NusA with protein N of phage lambda.
Bonin I, Mühlberger R, Bourenkov GP, Huber R, Bacher A, Richter G, Wahl MC
Proc Natl Acad Sci U S A 101 13762-7 (2004)
PMID: 15365170

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142713 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription termination/antitermination protein NusA Chains: A, B
Molecule details ›
Chains: A, B
Length: 70 amino acids
Theoretical weight: 7.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFF6 (Residues: 352-421; Coverage: 14%)
Gene names: JW3138, b3169, nusA
Structure domains: 5' to 3' exonuclease, C-terminal subdomain
Lambda N Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 813 Da
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand AU 1 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P43212
Unit cell:
a: 69.787Å b: 69.787Å c: 67.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.237
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Revisited gene regulation in bacteriophage lambda.
Dodd et al. (2005)
16 other articles

4 mentions without citation

Protein purification and crystallization artifacts: The tale usually not told.
Niedzialkowska et al. (2016)
3 more