› 1u9t

X-ray diffraction
2.16Å resolution

Crystal Structure Analysis of ChuS, an E. coli Heme Oxygenase

Released:
DOI: 10.2210/pdb1u9t/pdb
PDB entry 1u9t coloured by chain, front view.
PDB entry 1u9t coloured by chain, side view.
PDB entry 1u9t coloured by chain, top view.
Source organism: Escherichia coli O157:H7 str. EDL933
Primary publication:
Identification of an Escherichia coli O157:H7 heme oxygenase with tandem functional repeats.
Suits MD, Pal GP, Nakatsu K, Matte A, Cygler M, Jia Z
Proc Natl Acad Sci U S A 102 16955-60 (2005)
PMID: 16275907

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haemin-degrading HemS/ChuX domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.05 KDa
Source organism: Escherichia coli O157:H7 str. EDL933
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X5N8 (Residues: 1-342; Coverage: 100%)
Gene name: ECs_4379
Sequence domains: Haemin-degrading HemS.ChuX domain
Structure domains: HemS/ChuS/ChuX like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P21
Unit cell:
a: 41.684Å b: 57.88Å c: 59.578Å
α: 90° β: 96.02° γ: 90°
R-values:
R R work R free
0.207 0.207 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Overcoming the heme paradox: heme toxicity and tolerance in bacterial pathogens.
Anzaldi et al. (2010)
14 more

2 mentions without citation

Identification of an Escherichia coli O157:H7 heme oxygenase with tandem functional repeats.
Suits et al. (2005)
1 more