› 1ua7

X-ray diffraction
2.21Å resolution

Crystal Structure Analysis of Alpha-Amylase from Bacillus Subtilis complexed with Acarbose

Released:
DOI: 10.2210/pdb1ua7/pdb
PDB entry 1ua7 coloured by chain, front view.
PDB entry 1ua7 coloured by chain, side view.
PDB entry 1ua7 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.
Kagawa M, Fujimoto Z, Momma M, Takase K, Mizuno H
J Bacteriol 185 6981-4 (2003)
PMID: 14617662

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 46.8 KDa
Source organism: Bacillus subtilis
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P00691 (Residues: 45-466; Coverage: 67%)
Gene names: BSU03040, amyA, amyE
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GLD
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, G6D
Carbohydrate polymer
2 bound ligands:
Ligand ACI 2 x ACI
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P212121
Unit cell:
a: 70.32Å b: 74.204Å c: 115.724Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.265
Expression system: Bacillus subtilis

Quick links

Citations

14 citation in other articles

Crystal structure of a compact α-amylase from Geobacillus thermoleovorans.
Mok et al. (2013)
13 more

7 mentions without citation

Catalytically-active inclusion bodies for biotechnology-general concepts, optimization, and application.
Jäger et al. (2020)
6 more