1uc2

X-ray diffraction
2.15Å resolution

Hypothetical Extein Protein of PH1602 from Pyrococcus horikoshii

Released:
Model geometry
Fit model/data
Data not deposited

Function and Biology Details

Reaction catalysed:
(1a) GTP + [RNA ligase]-L-histidine = 5'-guanosyl [RNA ligase]-N(tau)-phosphono-L-histidine + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129838 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Pho hyp2 intein Chains: A, B
Molecule details ›
Chains: A, B
Length: 481 amino acids
Theoretical weight: 53.58 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O59245 (Residues: 406-486, 487-871; Coverage: 53%)
Gene names: PH1602, rtcB
Sequence domains: tRNA-splicing ligase RtcB
Structure domains: tRNA-splicing ligase RtcB

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 81.251Å b: 137.586Å c: 148.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.204
Expression system: Escherichia coli