› 1ucc

X-ray diffraction
1.77Å resolution

Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP.

Released:
DOI: 10.2210/pdb1ucc/pdb
PDB entry 1ucc coloured by chain, front view.
PDB entry 1ucc coloured by chain, side view.
PDB entry 1ucc coloured by chain, top view.
Source organism: Momordica charantia
Primary publication:
Crystal structures of the ribonuclease MC1 from bitter gourd seeds, complexed with 2'-UMP or 3'-UMP, reveal structural basis for uridine specificity.
Suzuki A, Yao M, Tanaka I, Numata T, Kikukawa S, Yamasaki N, Kimura M
Biochem Biophys Res Commun 275 572-6 (2000)
PMID: 10964705

Function and Biology Details

Reaction catalysed: 
(1a) RNA = an (RNA fragment)-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease MC Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.23 KDa
Source organism: Momordica charantia
UniProt:
  • Canonical: P23540 (Residues: 1-191; Coverage: 100%)
Gene name: MC1
Sequence domains: Ribonuclease T2 family
Structure domains: Ribonuclease T2-like

Ligands and Environments

1 bound ligand:
Ligand U3P 1 x U3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 38.8Å b: 67.63Å c: 75.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.214

Quick links

Citations

3 review citations

T2 Family ribonucleases: ancient enzymes with diverse roles.
Luhtala et al. (2010)
2 more

1 mention without citation

Variation among S-locus haplotypes and among stylar RNases in almond.
Goonetilleke et al. (2020)