› 1ugq

X-ray diffraction
2Å resolution

Crystal structure of apoenzyme of Co-type nitrile hydratase

Released:
DOI: 10.2210/pdb1ugq/pdb
Model geometry
Fit model/data
PDB entry 1ugq coloured by chain, front view.
PDB entry 1ugq coloured by chain, side view.
PDB entry 1ugq coloured by chain, top view.
Source organism: Pseudonocardia thermophila
Primary publication:
Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding.
Miyanaga A, Fushinobu S, Ito K, Shoun H, Wakagi T
Eur J Biochem 271 429-38 (2004)
PMID: 14717710

Function and Biology Details

Reaction catalysed: 
An aliphatic amide = a nitrile + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-181955 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cobalt-containing nitrile hydratase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 23.04 KDa
Source organism: Pseudonocardia thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SID2 (Residues: 2-204; Coverage: 100%)
Sequence domains: Nitrile hydratase, alpha chain
Structure domains: Nitrile hydratase alpha /Thiocyanate hydrolase gamma
Cobalt-containing nitrile hydratase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 228 amino acids
Theoretical weight: 26.17 KDa
Source organism: Pseudonocardia thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SID3 (Residues: 1-228; Coverage: 98%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P3221
Unit cell:
a: 65.362Å b: 65.362Å c: 184.099Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.22
Expression system: Escherichia coli

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Citations

1 review citation

Nitrile hydratase as a promising biocatalyst: recent advances and future prospects.
Feng et al. (2024)
30 other articles

4 mentions without citation

Recent Advances and Promises in Nitrile Hydratase: From Mechanism to Industrial Applications.
Cheng et al. (2020)
3 more