1ui0

X-ray diffraction
1.5Å resolution

Crystal Structure Of Uracil-DNA Glycosylase From Thermus Thermophilus HB8

Released:

Function and Biology Details

Reaction catalysed:
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-178079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-4 uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 23 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SKC5 (Residues: 1-205; Coverage: 100%)
Gene names: TTHA0718, udg
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P212121
Unit cell:
a: 45.429Å b: 59.512Å c: 80.971Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.201
Expression system: Escherichia coli BL21(DE3)