› 1uiy

X-ray diffraction
2.85Å resolution

Crystal Structure of Enoyl-CoA Hydratase from Thermus Thermophilus HB8

Released:
DOI: 10.2210/pdb1uiy/pdb
PDB entry 1uiy coloured by chain, front view.
PDB entry 1uiy coloured by chain, side view.
PDB entry 1uiy coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structure of enoyl-CoA hydratase from Thermus thermophilus HB8.
Padavattan S, Jos S, Gogoi H, Bagautdinov B
Acta Crystallogr F Struct Biol Commun 77 148-155 (2021)
PMID: 33949975

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160732 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative enoyl-CoA hydratase Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 27.36 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SKU3 (Residues: 1-253; Coverage: 100%)
Gene name: TTHA0550
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand DIO 1 x DIO
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: R32
Unit cell:
a: 131.233Å b: 131.233Å c: 110.608Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.267
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

'Omics-guided prediction of the pathway for metabolism of isoprene by Variovorax sp. WS11.
Dawson et al. (2022)

1 mention without citation

Crystal structure of enoyl-CoA hydratase from Thermus thermophilus HB8.
Padavattan et al. (2021)