› 1ujn

X-ray diffraction
1.8Å resolution

Crystal structure of dehydroquinate synthase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1ujn/pdb
PDB entry 1ujn coloured by chain, front view.
PDB entry 1ujn coloured by chain, side view.
PDB entry 1ujn coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of dehydroquinate synthase from Thermus thermophilus HB8 showing functional importance of the dimeric state.
Sugahara M, Nodake Y, Sugahara M, Kunishima N
Proteins 58 249-52 (2005)
PMID: 15508124

Function and Biology Details

Reaction catalysed: 
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160733 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-dehydroquinate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 37.56 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: P83703 (Residues: 1-348; Coverage: 100%)
Gene names: TTHN1_00272, aroB
Sequence domains: 3-dehydroquinate synthase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 63.403Å b: 71.787Å c: 71.636Å
α: 90° β: 99.44° γ: 90°
R-values:
R R work R free
0.203 0.202 0.236
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Crystal structure of novel NADP-dependent 3-hydroxyisobutyrate dehydrogenase from Thermus thermophilus HB8.
Lokanath et al. (2005)
6 more

6 mentions without citation

1,3-Propanediol dehydrogenase from Klebsiella pneumoniae: decameric quaternary structure and possible subunit cooperativity.
Marçal et al. (2009)
5 more