› 1ujz

X-ray diffraction
2.1Å resolution

Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein

Released:
DOI: 10.2210/pdb1ujz/pdb
PDB entry 1ujz coloured by chain, front view.
PDB entry 1ujz coloured by chain, side view.
PDB entry 1ujz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Computational redesign of protein-protein interaction specificity.
Kortemme T, Joachimiak LA, Bullock AN, Schuler AD, Stoddard BL, Baker D
Nat Struct Mol Biol 11 371-9 (2004)
PMID: 15034550

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169934 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E7 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03708 (Residues: 1-87; Coverage: 100%)
Gene names: ceiE7, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E7 Chain: B
Molecule details ›
Chain: B
Length: 128 amino acids
Theoretical weight: 14.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 446-573; Coverage: 22%)
Gene names: cea, colE7
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: I222
Unit cell:
a: 62.882Å b: 74.552Å c: 120.444Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.242 0.27
Expression system: Escherichia coli

Quick links

Citations

40 review citations

Macromolecular modeling with rosetta.
Das et al. (2008)
39 more

5 mentions without citation

The Importance of Non-accessible Crosslinks and Solvent Accessible Surface Distance in Modeling Proteins with Restraints From Crosslinking Mass Spectrometry.
Matthew Allen Bullock et al. (2016)
4 more