› 1ukf

X-ray diffraction
1.35Å resolution

Crystal Structure of Pseudomonas Avirulence Protein AvrPphB

Released:
DOI: 10.2210/pdb1ukf/pdb
PDB entry 1ukf coloured by chain, front view.
PDB entry 1ukf coloured by chain, side view.
PDB entry 1ukf coloured by chain, top view.
Source organism: Pseudomonas savastanoi pv. phaseolicola
Primary publication:
The crystal structure of Pseudomonas avirulence protein AvrPphB: a papain-like fold with a distinct substrate-binding site.
Zhu M, Shao F, Innes RW, Dixon JE, Xu Z
Proc Natl Acad Sci U S A 101 302-7 (2004)
PMID: 14694194

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
28 kDa product Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 20.39 KDa
Source organism: Pseudomonas savastanoi pv. phaseolicola
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q52430 (Residues: 81-267; Coverage: 70%)
Gene name: avrPph3
Sequence domains: Yersinia/Haemophilus virulence surface antigen
Structure domains: Cathepsin B; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 43.601Å b: 49.596Å c: 75.705Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Type III secretion system effector proteins: double agents in bacterial disease and plant defense.
Alfano et al. (2004)
28 more

21 mentions without citation

The Modes of Action of MARTX Toxin Effector Domains.
Kim BS. (2018)
20 more