› 1ukj

X-ray diffraction
1.8Å resolution

Detailed structure of L-Methionine-Lyase from Pseudomonas putida

Released:
DOI: 10.2210/pdb1ukj/pdb
PDB entry 1ukj coloured by chain, front view.
PDB entry 1ukj coloured by chain, side view.
PDB entry 1ukj coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Misaki S, Takimoto A, Takakura T, Yoshioka T, Yamashita M, Tamura T, Tanaka H, Inagaki K

Function and Biology Details

Reactions catalysed: 
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
(1a) L-homocysteine = hydrogen sulfide + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-methionine gamma-lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 398 amino acids
Theoretical weight: 42.9 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P13254 (Residues: 1-398; Coverage: 100%)
Gene name: mdeA
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU
Spacegroup: P43212
Unit cell:
a: 133.093Å b: 133.093Å c: 215.028Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.295
Expression system: Escherichia coli

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