› 1umv

X-ray diffraction
1.79Å resolution

Crystal structure of an acidic, non-myotoxic phospholipase A2 from the venom of Bothrops jararacussu

Released:
DOI: 10.2210/pdb1umv/pdb
PDB entry 1umv coloured by chain, front view.
PDB entry 1umv coloured by chain, side view.
PDB entry 1umv coloured by chain, top view.
Source organism: Bothrops jararacussu
Primary publication:
Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A2 in the monomeric and dimeric states: insights into its oligomeric state.
Magro AJ, Murakami MT, Marcussi S, Soares AM, Arni RK, Fontes MR
Biochem Biophys Res Commun 323 24-31 (2004)
PMID: 15351695

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acidic phospholipase A2 BthA-1 Chain: X
Molecule details ›
Chain: X
Length: 122 amino acids
Theoretical weight: 13.7 KDa
Source organism: Bothrops jararacussu
UniProt:
  • Canonical: Q8AXY1 (Residues: 17-138; Coverage: 100%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: C2221
Unit cell:
a: 39.99Å b: 53.99Å c: 90.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.229

Quick links

Citations

12 citation in other articles

Comparative structural studies on Lys49-phospholipases A(2) from Bothrops genus reveal their myotoxic site.
dos Santos et al. (2009)
11 more

2 mentions without citation

Relating destabilizing regions to known functional sites in proteins.
Dessailly et al. (2007)
1 more