› 1un2

X-ray diffraction
2.4Å resolution

Crystal structure of circularly permuted CPDSBA_Q100T99: Preserved Global Fold and Local Structural Adjustments

Released:
DOI: 10.2210/pdb1un2/pdb
PDB entry 1un2 coloured by chain, front view.
PDB entry 1un2 coloured by chain, side view.
PDB entry 1un2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of circularly permuted DsbA(Q100T99): preserved global fold and local structural adjustments.
Manjasetty BA, Hennecke J, Glockshuber R, Heinemann U
Acta Crystallogr D Biol Crystallogr 60 304-9 (2004)
PMID: 14747707

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol:disulfide interchange protein DsbA Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 21.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEG4 (Residues: 20-118; Coverage: 52%)
Gene names: JW3832, b3860, dsbA, dsf, ppfA
Sequence domains: DSBA-like thioredoxin domain
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 37.76Å b: 52.59Å c: 53.73Å
α: 90° β: 103.23° γ: 90°
R-values:
R R work R free
0.211 0.206 0.256
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Circular permutation: a different way to engineer enzyme structure and function.
Yu et al. (2011)
3 other articles

1 mention without citation

CPSARST: an efficient circular permutation search tool applied to the detection of novel protein structural relationships.
Lo et al. (2008)