› 1unf

X-ray diffraction
1.97Å resolution

The crystal structure of the eukaryotic FeSOD from Vigna unguiculata suggests a new enzymatic mechanism

Released:
DOI: 10.2210/pdb1unf/pdb
PDB entry 1unf coloured by chain, front view.
PDB entry 1unf coloured by chain, side view.
PDB entry 1unf coloured by chain, top view.
Source organism: Vigna unguiculata
Primary publication:
The crystal structure of an eukaryotic iron superoxide dismutase suggests intersubunit cooperation during catalysis.
Muñoz IG, Moran JF, Becana M, Montoya G
Protein Sci 14 387-94 (2005)
PMID: 15659371

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase Chain: X
Molecule details ›
Chain: X
Length: 238 amino acids
Theoretical weight: 26.74 KDa
Source organism: Vigna unguiculata
UniProt:
  • Canonical: Q9M7R2 (Residues: 8-245; Coverage: 97%)
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 81.985Å b: 48.163Å c: 63.671Å
α: 90° β: 119.76° γ: 90°
R-values:
R R work R free
0.15 0.148 0.192

Quick links

Citations

3 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
2 more

6 mentions without citation

Structures of native and Fe-substituted SOD2 from Saccharomyces cerevisiae.
Kang et al. (2011)
5 more