› 1up6

X-ray diffraction
2.55Å resolution

Structure of the 6-phospho-beta glucosidase from Thermotoga maritima at 2.55 Angstrom resolution in the tetragonal form with manganese, NAD+ and glucose-6-phosphate

Released:
DOI: 10.2210/pdb1up6/pdb
PDB entry 1up6 coloured by chain, front view.
PDB entry 1up6 coloured by chain, side view.
PDB entry 1up6 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima.
Yip VL, Varrot A, Davies GJ, Rajan SS, Yang X, Thompson J, Anderson WF, Withers SG
J Am Chem Soc 126 8354-5 (2004)
PMID: 15237973

Function and Biology Details

Reaction catalysed: 
6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194761 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phospho-beta-glucosidase BglT Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 416 amino acids
Theoretical weight: 48.16 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X108 (Residues: 1-415; Coverage: 100%)
Gene names: TM_1281, bglT
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 3 x NAD
3 bound ligands:
Ligand MN 5 x MN
Ligand G6P 2 x G6P
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 56 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 179.438Å b: 179.438Å c: 282.911Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.25
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
17 more

2 mentions without citation

The sim operon facilitates the transport and metabolism of sucrose isomers in Lactobacillus casei ATCC 334.
Thompson et al. (2008)
1 more