› 1up7

X-ray diffraction
2.4Å resolution

Structure of the 6-phospho-beta glucosidase from Thermotoga maritima at 2.4 Angstrom resolution in the tetragonal form with NAD and glucose-6-phosphate

Released:
DOI: 10.2210/pdb1up7/pdb
PDB entry 1up7 coloured by chain, front view.
PDB entry 1up7 coloured by chain, side view.
PDB entry 1up7 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
NAD+ and metal-ion dependent hydrolysis by family 4 glycosidases: structural insight into specificity for phospho-beta-D-glucosides.
Varrot A, Yip VL, Li Y, Rajan SS, Yang X, Anderson WF, Thompson J, Withers SG, Davies GJ
J Mol Biol 346 423-35 (2005)
PMID: 15670594

Function and Biology Details

Reaction catalysed: 
6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194761 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phospho-beta-glucosidase BglT Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 417 amino acids
Theoretical weight: 47.99 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X108 (Residues: 1-415; Coverage: 100%)
Gene names: TM_1281, bglT
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
2 bound ligands:
Ligand G6P 8 x G6P
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 178.132Å b: 178.132Å c: 278.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.24
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Mechanistic insights into glycosidase chemistry.
Vocadlo et al. (2008)
7 more

1 mention without citation

Unveiling lignocellulolytic trait of a goat omasum inhabitant Klebsiella variicola strain HSTU-AAM51 in light of biochemical and genome analyses.
Md et al. (2022)