› 1upn

Electron Microscopy
16Å resolution

COMPLEX OF ECHOVIRUS TYPE 12 WITH DOMAINS 3 AND 4 OF ITS RECEPTOR DECAY ACCELERATING FACTOR (CD55) BY CRYO ELECTRON MICROSCOPY AT 16 A

Released:
DOI: 10.2210/pdb1upn/pdb
PDB entry 1upn coloured by chain, front view.
PDB entry 1upn coloured by chain, side view.
PDB entry 1upn coloured by chain, top view.
Source organisms:
Primary publication:
The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55).
Bhella D, Goodfellow IG, Roversi P, Pettigrew D, Chaudhry Y, Evans DJ, Lea SM
J Biol Chem 279 8325-32 (2004)
PMID: 14634014
Related structures: EMD-1057

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139976 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
ECHOVIRUS 11 COAT PROTEIN VP1 Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 32.79 KDa
Source organism: Echovirus E11
UniProt:
  • Canonical: Q8JKE8 (Residues: 570-861; Coverage: 13%)
Sequence domains: picornavirus capsid protein
ECHOVIRUS 11 COAT PROTEIN VP2 Chain: B
Molecule details ›
Chain: B
Length: 262 amino acids
Theoretical weight: 29.05 KDa
Source organism: Echovirus E11
UniProt:
  • Canonical: Q8JKE8 (Residues: 70-331; Coverage: 12%)
Sequence domains: picornavirus capsid protein
ECHOVIRUS 11 COAT PROTEIN VP3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 25.9 KDa
Source organism: Echovirus E11
UniProt:
  • Canonical: Q8JKE8 (Residues: 332-569; Coverage: 11%)
Sequence domains: picornavirus capsid protein
ECHOVIRUS 11 COAT PROTEIN VP4 Chain: D
Molecule details ›
Chain: D
Length: 69 amino acids
Theoretical weight: 7.51 KDa
Source organism: Echovirus E11
UniProt:
  • Canonical: Q8JKE8 (Residues: 1-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
Complement decay-accelerating factor Chain: E
Molecule details ›
Chain: E
Length: 129 amino acids
Theoretical weight: 14.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08174 (Residues: 157-285; Coverage: 37%)
Gene names: CD55, CR, DAF
Sequence domains: Sushi repeat (SCR repeat)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 16Å
Relevant EMDB volumes: EMD-1057
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Blood Groups in Infection and Host Susceptibility.
Cooling L. (2015)
18 other articles

4 mentions without citation

Mol* Viewer: modern web app for 3D visualization and analysis of large biomolecular structures.
Sehnal et al. (2021)
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