› 1ups

X-ray diffraction
1.82Å resolution

GlcNAc[alpha]1-4Gal releasing endo-[beta]-galactosidase from Clostridium perfringens

Released:
DOI: 10.2210/pdb1ups/pdb
PDB entry 1ups coloured by chain, front view.
PDB entry 1ups coloured by chain, side view.
PDB entry 1ups coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Three-dimensional structure of GlcNAcalpha1-4Gal releasing endo-beta-galactosidase from Clostridium perfringens.
Tempel W, Liu ZJ, Horanyi PS, Deng L, Lee D, Newton MG, Rose JP, Ashida H, Li SC, Li YT, Wang BC
Proteins 59 141-4 (2005)
PMID: 15688452

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH16 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 420 amino acids
Theoretical weight: 49.43 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q934G8 (Residues: 1-420; Coverage: 100%)
Gene name: gngC
Sequence domains: Glycosyl hydrolases family 16
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P63
Unit cell:
a: 160.43Å b: 160.43Å c: 86.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.176 0.204
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bioconversion of red seaweed galactans: a focus on bacterial agarases and carrageenases.
Michel et al. (2006)
3 other articles

6 mentions without citation

Structural and functional insights into the B30.2/SPRY domain.
Woo et al. (2006)
5 more