› 1uq5

X-ray diffraction
1.4Å resolution

RICIN A-CHAIN (RECOMBINANT) N122A MUTANT

Released:
DOI: 10.2210/pdb1uq5/pdb
PDB entry 1uq5 coloured by chain, front view.
PDB entry 1uq5 coloured by chain, side view.
PDB entry 1uq5 coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain.
Marsden CJ, Fülöp V, Day PJ, Lord JM
Eur J Biochem 271 153-62 (2004)
PMID: 14686928

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.41 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02879 (Residues: 40-302; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P41212
Unit cell:
a: 67.5Å b: 67.5Å c: 140.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.214
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Vaccines against the category B toxins: Staphylococcal enterotoxin B, epsilon toxin and ricin.
Mantis NJ. (2005)
3 more

1 mention without citation

Ribosome Inactivating Proteins from Rosaceae.
Shang et al. (2016)