› 1us2

X-ray diffraction
1.85Å resolution

Xylanase10C (mutant E385A) from Cellvibrio japonicus in complex with xylopentaose

Released:
DOI: 10.2210/pdb1us2/pdb
PDB entry 1us2 coloured by chain, front view.
PDB entry 1us2 coloured by chain, side view.
PDB entry 1us2 coloured by chain, top view.
Source organism: Cellvibrio japonicus
Primary publication:
Structural and biochemical analysis of Cellvibrio japonicus xylanase 10C: how variation in substrate-binding cleft influences the catalytic profile of family GH-10 xylanases.
Pell G, Szabo L, Charnock SJ, Xie H, Gloster TM, Davies GJ, Gilbert HJ
J Biol Chem 279 11777-88 (2004)
PMID: 14670951

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endo-beta-1,4-xylanase Xyn10C Chain: A
Molecule details ›
Chain: A
Length: 530 amino acids
Theoretical weight: 58.15 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59675 (Residues: 86-606; Coverage: 89%)
Gene names: xyn10C, xynF
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 44.597Å b: 82.686Å c: 170.727Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.226
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Xylanases, xylanase families and extremophilic xylanases.
Collins et al. (2005)
5 more

7 mentions without citation

Mapping glycoside hydrolase substrate subsites by isothermal titration calorimetry.
Zolotnitsky et al. (2004)
6 more