› 1us4

X-ray diffraction
1.75Å resolution

PUTATIVE GLUR0 LIGAND BINDING CORE WITH L-GLUTAMATE

Released:
DOI: 10.2210/pdb1us4/pdb
PDB entry 1us4 coloured by chain, front view.
PDB entry 1us4 coloured by chain, side view.
PDB entry 1us4 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structure of the Thermus thermophilus putative periplasmic glutamate/glutamine-binding protein.
Takahashi H, Inagaki E, Kuroishi C, Tahirov TH
Acta Crystallogr D Biol Crystallogr 60 1846-54 (2004)
PMID: 15388932

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 33.72 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83817 (Residues: 1-314; Coverage: 100%)
Sequence domains: NMT1-like family
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand GLU 1 x GLU
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 51.518Å b: 68.092Å c: 81.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.206
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Tripartite ATP-independent periplasmic (TRAP) transporters in bacteria and archaea.
Mulligan et al. (2011)
1 more

3 mentions without citation

Tripartite ATP-Independent Periplasmic (TRAP) Transporters and Tripartite Tricarboxylate Transporters (TTT): From Uptake to Pathogenicity.
Rosa et al. (2018)
2 more