› 1usl

X-ray diffraction
1.88Å resolution

Structure Of Mycobacterium tuberculosis Ribose-5-Phosphate Isomerase, RpiB, Rv2465c, Complexed With Phosphate.

Released:
DOI: 10.2210/pdb1usl/pdb
PDB entry 1usl coloured by chain, front view.
PDB entry 1usl coloured by chain, side view.
PDB entry 1usl coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Mycobacterium tuberculosis ribose-5-phosphate isomerase has a known fold, but a novel active site.
Roos AK, Andersson CE, Bergfors T, Jacobsson M, Karlén A, Unge T, Jones TA, Mowbray SL
J Mol Biol 335 799-809 (2004)
PMID: 14687575

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase B Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 170 amino acids
Theoretical weight: 18.33 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WKD7 (Residues: 1-162; Coverage: 100%)
Gene names: MTV008.21c, Rv2465c, rpiB
Sequence domains: Ribose/Galactose Isomerase
Structure domains: Sugar-phosphate isomerase, RpiB/LacA/LacB

Ligands and Environments

1 bound ligand:
Ligand PO4 5 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2
Unit cell:
a: 136.42Å b: 102.39Å c: 69.61Å
α: 90° β: 95.68° γ: 90°
R-values:
R R work R free
0.17 0.168 0.202
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Lipoarabinomannan and related glycoconjugates: structure, biogenesis and role in Mycobacterium tuberculosis physiology and host-pathogen interaction.
Mishra et al. (2011)
1 more

3 mentions without citation

Protein surface analysis for function annotation in high-throughput structural genomics pipeline.
Binkowski et al. (2005)
2 more