› 1uso

X-ray diffraction
1.3Å resolution

DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR, PRO9LEU MUTANT

Released:
DOI: 10.2210/pdb1uso/pdb
PDB entry 1uso coloured by chain, front view.
PDB entry 1uso coloured by chain, side view.
PDB entry 1uso coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Tahirov TH, Inagaki E

Function and Biology Details

Reaction catalysed: 
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177859 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4a-hydroxytetrahydrobiopterin dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 80 amino acids
Theoretical weight: 9.49 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SHC7 (Residues: 1-80; Coverage: 100%)
Gene name: TTHA1803
Sequence domains: Pterin 4 alpha carbinolamine dehydratase
Structure domains: Transcriptional coactivator/pterin dehydratase

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 31.43Å b: 48.687Å c: 94.021Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.238
Expression system: Escherichia coli BL21(DE3)

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