› 1usw

X-ray diffraction
2.5Å resolution

Crystal Structure of Ferulic Acid Esterase from Aspergillus niger

Released:
DOI: 10.2210/pdb1usw/pdb
PDB entry 1usw coloured by chain, front view.
PDB entry 1usw coloured by chain, side view.
PDB entry 1usw coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
The crystal structure of feruloyl esterase A from Aspergillus niger suggests evolutive functional convergence in feruloyl esterase family.
Hermoso JA, Sanz-Aparicio J, Molina R, Juge N, González R, Faulds CB
J Mol Biol 338 495-506 (2004)
PMID: 15081808

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Feruloyl esterase A Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 28.36 KDa
Source organism: Aspergillus niger
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O42807 (Residues: 22-281; Coverage: 100%)
Gene name: faeA
Sequence domains: Lipase (class 3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P21
Unit cell:
a: 39.2Å b: 47.18Å c: 62.4Å
α: 90° β: 96.14° γ: 90°
R-values:
R R work R free
0.212 0.212 0.294
Expression system: Komagataella pastoris

Quick links

Citations

9 review citations

Ferulic acid: an antioxidant found naturally in plant cell walls and feruloyl esterases involved in its release and their applications.
Mathew et al. (2004)
8 more

8 mentions without citation

Genomics review of holocellulose deconstruction by aspergilli.
Segato et al. (2014)
7 more