› 1ut2

X-ray diffraction
3.3Å resolution

AfaE-3 adhesin from Escherichia Coli

Released:
DOI: 10.2210/pdb1ut2/pdb
PDB entry 1ut2 coloured by chain, front view.
PDB entry 1ut2 coloured by chain, side view.
PDB entry 1ut2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
High resolution studies of the Afa/Dr adhesin DraE and its interaction with chloramphenicol.
Pettigrew D, Anderson KL, Billington J, Cota E, Simpson P, Urvil P, Rabuzin F, Roversi P, Nowicki B, du Merle L, Le Bouguénec C, Matthews S, Lea SM
J Biol Chem 279 46851-7 (2004)
PMID: 15331605

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Afimbrial adhesin AFA-III Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 149 amino acids
Theoretical weight: 16.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57254 (Residues: 20-160; Coverage: 100%)
Gene names: afaE-3, afaE3
Sequence domains: Dr-family adhesin
Structure domains: Dr adhesin

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 113.295Å b: 113.295Å c: 164.125Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.226 0.266
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek.
Nuccio et al. (2007)
13 more

2 mentions without citation

Crystallization and initial crystallographic analysis of AafA: the major adhesive subunit of the enteroaggregative Escherichia coli AAF/II pilus.
Yang et al. (2011)
1 more