› 1utd

X-ray diffraction
2.1Å resolution

The structure of the trp RNA-binding attenuation protein (TRAP) bound to a 63-nucleotide RNA molecule containing GAGUUU repeats

Released:
DOI: 10.2210/pdb1utd/pdb
PDB entry 1utd coloured by chain, front view.
PDB entry 1utd coloured by chain, side view.
PDB entry 1utd coloured by chain, top view.
Source organisms:
Primary publication:
The interaction of RNA with TRAP: the role of triplet repeats and separating spacer nucleotides.
Hopcroft NH, Manfredo A, Wendt AL, Brzozowski AM, Gollnick P, Antson AA
J Mol Biol 338 43-53 (2004)
PMID: 15050822

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo undecamer
hetero 22-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194935 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription attenuation protein MtrB Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V
Length: 74 amino acids
Theoretical weight: 8.26 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X6J6 (Residues: 1-74; Coverage: 100%)
Gene name: mtrB
Sequence domains: Tryptophan RNA-binding attenuator protein
Structure domains: TRAP-like
5'-R(*GP*UP*UP*UP*GP*AP)-3' Chains: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, Z
Molecule details ›
Chains: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, Z
Length: 6 nucleotides
Theoretical weight: 1.89 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRP 22 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 115.11Å b: 134.23Å c: 119.74Å
α: 90° β: 106.45° γ: 90°
R-values:
R R work R free
0.17 0.169 0.228
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Complexity in regulation of tryptophan biosynthesis in Bacillus subtilis.
Gollnick et al. (2005)
1 more