› 1uty

X-ray diffraction
2.4Å resolution

Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)

Released:
DOI: 10.2210/pdb1uty/pdb
PDB entry 1uty coloured by chain, front view.
PDB entry 1uty coloured by chain, side view.
PDB entry 1uty coloured by chain, top view.
Source organism: Bluetongue virus 8
Primary publication:
Structure and assembly of the RNA binding domain of bluetongue virus non-structural protein 2.
Butan C, Van Der Zandt H, Tucker PA
J Biol Chem 279 37613-21 (2004)
PMID: 15155766

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural protein NS2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 187 amino acids
Theoretical weight: 21.36 KDa
Source organism: Bluetongue virus 8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23065 (Residues: 1-177, 178-182; Coverage: 51%)
Gene name: Segment-8
Sequence domains: Bluetongue virus non-structural protein NS2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P65
Unit cell:
a: 102.296Å b: 102.296Å c: 77.915Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.211 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Functional mapping of bluetongue virus proteins and their interactions with host proteins during virus replication.
Roy P. (2008)
2 more

2 mentions without citation

Role of NS2 specific RNA binding and phosphorylation in liquid-liquid phase separation and virus assembly.
Rahman et al. (2022)
1 more