› 1uum

X-ray diffraction
2.3Å resolution

Rat dihydroorotate dehydrogenase (DHOD)in complex with atovaquone

Released:
DOI: 10.2210/pdb1uum/pdb
PDB entry 1uum coloured by chain, front view.
PDB entry 1uum coloured by chain, side view.
PDB entry 1uum coloured by chain, top view.
Source organism: Rattus rattus
Primary publication:
Inhibitor binding in a class 2 dihydroorotate dehydrogenase causes variations in the membrane-associated N-terminal domain.
Hansen M, Le Nours J, Johansson E, Antal T, Ullrich A, Löffler M, Larsen S
Protein Sci 13 1031-42 (2004)
PMID: 15044733

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + a quinone = orotate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179032 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (quinone), mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 40.34 KDa
Source organism: Rattus rattus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q63707 (Residues: 30-395; Coverage: 93%)
Gene name: Dhodh
Sequence domains: Dihydroorotate dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
3 bound ligands:
Ligand ORO 2 x ORO
Ligand AFI 2 x AFI
Ligand BOG 2 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P3
Unit cell:
a: 133.15Å b: 133.15Å c: 50.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.216 0.242
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Chemistry and biology of multicomponent reactions.
Dömling et al. (2012)
1 more

3 mentions without citation

F901318 represents a novel class of antifungal drug that inhibits dihydroorotate dehydrogenase.
Oliver et al. (2016)
2 more