› 1uup

X-ray diffraction
2.6Å resolution

Crystal Structure of A Dimeric Form of Streptococcal Pyrogenic Exotoxin A (SpeA1).

Released:
DOI: 10.2210/pdb1uup/pdb
PDB entry 1uup coloured by chain, front view.
PDB entry 1uup coloured by chain, side view.
PDB entry 1uup coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Crystal structure of a dimeric form of streptococcal pyrogenic exotoxin A (SpeA1).
Baker MD, Gendlina I, Collins CM, Acharya KR
Protein Sci 13 2285-90 (2004)
PMID: 15295110

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exotoxin type A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 221 amino acids
Theoretical weight: 25.82 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DJY7 (Residues: 34-169, 170-250; Coverage: 99%)
Gene name: speA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P212121
Unit cell:
a: 53.3Å b: 127.2Å c: 148Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.282
Expression system: Escherichia coli BL21

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Citations

5 review citations

Gram-positive bacterial superantigen outside-in signaling causes toxic shock syndrome.
Brosnahan et al. (2011)
4 more

1 mention without citation

Superantigen Recognition and Interactions: Functions, Mechanisms and Applications.
Deacy et al. (2021)