› 1uv6

X-ray diffraction
2.5Å resolution

X-ray structure of acetylcholine binding protein (AChBP) in complex with carbamylcholine

Released:
DOI: 10.2210/pdb1uv6/pdb
PDB entry 1uv6 coloured by chain, front view.
PDB entry 1uv6 coloured by chain, side view.
PDB entry 1uv6 coloured by chain, top view.
Source organism: Lymnaea stagnalis
Primary publication:
Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures.
Celie PH, van Rossum-Fikkert SE, van Dijk WJ, Brejc K, Smit AB, Sixma TK
Neuron 41 907-14 (2004)
PMID: 15046723

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylcholine-binding protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 210 amino acids
Theoretical weight: 23.86 KDa
Source organism: Lymnaea stagnalis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P58154 (Residues: 20-229; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

1 bound ligand:
Ligand CCE 3 x CCE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 140.9Å b: 140.9Å c: 240.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.265
Expression system: Spodoptera frugiperda

Quick links

Citations

88 review citations

Mammalian nicotinic acetylcholine receptors: from structure to function.
Albuquerque et al. (2009)
87 more

64 mentions without citation

5-HT(3) receptors.
Lummis SC. (2012)
63 more