› 1uvk

X-ray diffraction
2.45Å resolution

The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 dead-end complex

Released:
DOI: 10.2210/pdb1uvk/pdb
PDB entry 1uvk coloured by chain, front view.
PDB entry 1uvk coloured by chain, side view.
PDB entry 1uvk coloured by chain, top view.
Source organism: Pseudomonas virus phi6
Primary publication:
The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase.
Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM
Structure 12 307-16 (2004)
PMID: 14962391

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 664 amino acids
Theoretical weight: 74.9 KDa
Source organism: Pseudomonas virus phi6
Expression system: Escherichia coli
UniProt:
  • Canonical: P11124 (Residues: 2-665; Coverage: 100%)
Gene name: P2
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MN 3 x MN
Ligand MG 6 x MG
Ligand POP 3 x POP
Ligand G 3 x G
Ligand GTP 3 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 106.29Å b: 93.8Å c: 140.77Å
α: 90° β: 101.35° γ: 90°
R-values:
R R work R free
0.243 0.243 0.265
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure-function relationships among RNA-dependent RNA polymerases.
Ng et al. (2008)
6 more

4 mentions without citation

RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution.
Venkataraman et al. (2018)
3 more