› 1ux5

X-ray diffraction
2.5Å resolution

Crystal Structures of a Formin Homology-2 domain reveal a flexibly tethered dimer architecture

Released:
DOI: 10.2210/pdb1ux5/pdb
PDB entry 1ux5 coloured by chain, front view.
PDB entry 1ux5 coloured by chain, side view.
PDB entry 1ux5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structures of a Formin Homology-2 domain reveal a tethered dimer architecture.
Xu Y, Moseley JB, Sagot I, Poy F, Pellman D, Goode BL, Eck MJ
Cell 116 711-23 (2004)
PMID: 15006353

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein BNI1 Chain: A
Molecule details ›
Chain: A
Length: 411 amino acids
Theoretical weight: 47.4 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P41832 (Residues: 1350-1760; Coverage: 21%)
Gene names: BNI1, N0646, PPF3, SHE5, YNL271C
Sequence domains: Formin Homology 2 Domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1, CHESS BEAMLINE F1, CHESS BEAMLINE F2
Spacegroup: P6122
Unit cell:
a: 101.4Å b: 101.4Å c: 265.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.207 0.251
Expression system: Escherichia coli

Quick links

Citations

50 review citations

Regulation of actin filament assembly by Arp2/3 complex and formins.
Pollard TD. (2007)
49 more

10 mentions without citation

Review of the mechanism of processive actin filament elongation by formins.
Paul et al. (2009)
9 more