› 1uza

X-ray diffraction
1.5Å resolution

Crystallographic structure of a feruloyl esterase from Aspergillus niger

Released:
DOI: 10.2210/pdb1uza/pdb
PDB entry 1uza coloured by chain, front view.
PDB entry 1uza coloured by chain, side view.
PDB entry 1uza coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Structure of a feruloyl esterase from Aspergillus niger.
McAuley KE, Svendsen A, Patkar SA, Wilson KS
Acta Crystallogr D Biol Crystallogr 60 878-87 (2004)
PMID: 15103133

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Feruloyl esterase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 28.47 KDa
Source organism: Aspergillus niger
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: O42807 (Residues: 22-281; Coverage: 100%)
Gene name: faeA
Sequence domains: Lipase (class 3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P1
Unit cell:
a: 38.298Å b: 39.681Å c: 77.071Å
α: 75.24° β: 78.82° γ: 71.3°
R-values:
R R work R free
0.158 0.156 0.188
Expression system: Aspergillus oryzae

Quick links

Citations

3 review citations

Occurrence, properties, and applications of feruloyl esterases.
Koseki et al. (2009)
2 more

1 mention without citation

Insights into substrate binding of ferulic acid esterases by arabinose and methyl hydroxycinnamate esters and molecular docking.
Hunt et al. (2017)