› 1uzb

X-ray diffraction
1.4Å resolution

1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE

Released:
DOI: 10.2210/pdb1uzb/pdb
PDB entry 1uzb coloured by chain, front view.
PDB entry 1uzb coloured by chain, side view.
PDB entry 1uzb coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of Thermus thermophilus Delta1-pyrroline-5-carboxylate dehydrogenase.
Inagaki E, Ohshima N, Takahashi H, Kuroishi C, Yokoyama S, Tahirov TH
J Mol Biol 362 490-501 (2006)
PMID: 16934832

Function and Biology Details

Reaction catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-glutamate gamma-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 516 amino acids
Theoretical weight: 57.11 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83849 (Residues: 1-516; Coverage: 100%)
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MRD 6 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: R3
Unit cell:
a: 102.285Å b: 102.285Å c: 279.283Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.177 0.19
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Structural biology of proline catabolism.
Tanner JJ. (2008)
5 more

5 mentions without citation

Substrate channeling in proline metabolism.
Arentson et al. (2012)
4 more