› 1uzu

X-ray diffraction
2.3Å resolution

Glycogen Phosphorylase b in complex with indirubin-5'-sulphonate

Released:
DOI: 10.2210/pdb1uzu/pdb
Model geometry
Fit model/data
PDB entry 1uzu coloured by chain, front view.
PDB entry 1uzu coloured by chain, side view.
PDB entry 1uzu coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Binding of the potential antitumour agent indirubin-5-sulphonate at the inhibitor site of rabbit muscle glycogen phosphorylase b. Comparison with ligand binding to pCDK2-cyclin A complex.
Kosmopoulou MN, Leonidas DD, Chrysina ED, Bischler N, Eisenbrand G, Sakarellos CE, Pauptit R, Oikonomakos NG
Eur J Biochem 271 2280-90 (2004)
PMID: 15153119

Function and Biology Details

Reaction catalysed: 
[(1->4)-alpha-D-glucosyl](n) + phosphate = [(1->4)-alpha-D-glucosyl](n-1)+ alpha-D-glucose 1-phosphate.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen phosphorylase, muscle form Chain: A
Molecule details ›
Chain: A
Length: 842 amino acids
Theoretical weight: 97.29 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P00489 (Residues: 2-843; Coverage: 100%)
Gene name: PYGM
Sequence domains: Carbohydrate phosphorylase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand INR 1 x INR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU
Spacegroup: P43212
Unit cell:
a: 128.652Å b: 128.652Å c: 116.389Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.214

Quick links

Citations

3 review citations

Tetrazoles via Multicomponent Reactions.
Neochoritis et al. (2019)
2 more

1 mention without citation

Prediction of Ordered Water Molecules in Protein Binding Sites from Molecular Dynamics Simulations: The Impact of Ligand Binding on Hydration Networks.
Rudling et al. (2018)