Function and Biology Details
Reactions catalysed:
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H(2)O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assemblies composition:
Assembly name:
Dhurrinase (preferred)
PDBe Complex ID:
PDB-CPX-174944 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dhurrinase
Molecule details ›
Chains: A, B, C, D, F
Length: 565 amino acids
Theoretical weight: 63.93 KDa
Source organism: Sorghum bicolor
Expression system: Escherichia coli
UniProt:
Structure domains: Glycosidases
Length: 565 amino acids
Theoretical weight: 63.93 KDa
Source organism: Sorghum bicolor
Expression system: Escherichia coli
UniProt:
- Canonical:
Q41290 (Residues: 1-565; Coverage: 100%)
Structure domains: Glycosidases
Dhurrinase
Molecule details ›
Chain: E
Length: 565 amino acids
Theoretical weight: 63.96 KDa
Source organism: Sorghum bicolor
Expression system: Escherichia coli
UniProt:
Structure domains: Glycosidases
Length: 565 amino acids
Theoretical weight: 63.96 KDa
Source organism: Sorghum bicolor
Expression system: Escherichia coli
UniProt:
- Canonical: Q41290 (Residues: 1-565; Coverage: 100%)
Structure domains: Glycosidases
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID14-1
Spacegroup:
P31
Expression system: Escherichia coli
