› 1v0a

X-ray diffraction
1.98Å resolution

Family 11 Carbohydrate-Binding Module of cellulosomal cellulase Lic26A-Cel5E of Clostridium thermocellum

Released:
DOI: 10.2210/pdb1v0a/pdb
PDB entry 1v0a coloured by chain, front view.
PDB entry 1v0a coloured by chain, side view.
PDB entry 1v0a coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates beta-1,4- and beta-1,3-1,4-mixed linked glucans at a single binding site.
Carvalho AL, Goyal A, Prates JA, Bolam DN, Gilbert HJ, Pires VM, Ferreira LM, Planas A, Romão MJ, Fontes CM
J Biol Chem 279 34785-93 (2004)
PMID: 15192099

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase H Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 20.07 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli
UniProt:
  • Canonical: P16218 (Residues: 652-821; Coverage: 20%)
Gene names: Cthe_1472, celH
Sequence domains: Carbohydrate binding domain (family 11)
Structure domains: Galactose-binding lectin

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21212
Unit cell:
a: 75.143Å b: 50.892Å c: 40.868Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.232
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.
Fontes et al. (2010)
3 more

4 mentions without citation

Substrate recognition and hydrolysis by a family 50 exo-β-agarase, Aga50D, from the marine bacterium Saccharophagus degradans.
Pluvinage et al. (2013)
3 more