› 1v3h

X-ray diffraction
1.6Å resolution

The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase

Released:
DOI: 10.2210/pdb1v3h/pdb
PDB entry 1v3h coloured by chain, front view.
PDB entry 1v3h coloured by chain, side view.
PDB entry 1v3h coloured by chain, top view.
Source organism: Glycine max
Primary publication:
The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase.
Kang YN, Adachi M, Utsumi S, Mikami B
J Mol Biol 339 1129-40 (2004)
PMID: 15178253

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145335 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-amylase Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 55.96 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: P10538 (Residues: 2-496; Coverage: 100%)
Gene name: BMY1
Sequence domains: Glycosyl hydrolase family 14
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P3121
Unit cell:
a: 85.379Å b: 85.379Å c: 143.959Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.163 0.181
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts.
Fulton et al. (2008)
7 more