› 1v4e

X-ray diffraction
2.28Å resolution

Crystal Structure of Octaprenyl Pyrophosphate Synthase from Hyperthermophilic Thermotoga maritima

Released:
DOI: 10.2210/pdb1v4e/pdb
PDB entry 1v4e coloured by chain, front view.
PDB entry 1v4e coloured by chain, side view.
PDB entry 1v4e coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Crystal structure of octaprenyl pyrophosphate synthase from hyperthermophilic Thermotoga maritima and mechanism of product chain length determination.
Guo RT, Kuo CJ, Chou CC, Ko TP, Shr HL, Liang PH, Wang AH
J Biol Chem 279 4903-12 (2004)
PMID: 14617622

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-194821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Octoprenyl-diphosphate synthase, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 33.9 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X1M1 (Residues: 1-299; Coverage: 100%)
Gene name: TM_1535
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P4212
Unit cell:
a: 151.53Å b: 151.53Å c: 69.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Structural and Chemical Biology of Terpenoid Cyclases.
Christianson DW. (2017)
10 more

8 mentions without citation

Terpenoid synthase structures: a so far incomplete view of complex catalysis.
Gao et al. (2012)
7 more