› 1v4v

X-ray diffraction
1.8Å resolution

Crystal Structure Of UDP-N-Acetylglucosamine 2-Epimerase From Thermus Thermophilus HB8

Released:
DOI: 10.2210/pdb1v4v/pdb
PDB entry 1v4v coloured by chain, front view.
PDB entry 1v4v coloured by chain, side view.
PDB entry 1v4v coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Bagautdinov B, Tahirov TH, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) Chains: A, B
Molecule details ›
Chains: A, B
Length: 376 amino acids
Theoretical weight: 41.4 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83824 (Residues: 1-376; Coverage: 100%)
Sequence domains: UDP-N-acetylglucosamine 2-epimerase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand ACY 1 x ACY
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 169.728Å b: 51.448Å c: 105.506Å
α: 90° β: 104.2° γ: 90°
R-values:
R R work R free
0.209 0.209 0.235
Expression system: Escherichia coli BL21(DE3)

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