› 1v6t

X-ray diffraction
1.7Å resolution

Crystal Structure of Lactam Utilization Protein from Pyrococcus horikoshii Ot3

Released:
DOI: 10.2210/pdb1v6t/pdb
PDB entry 1v6t coloured by chain, front view.
PDB entry 1v6t coloured by chain, side view.
PDB entry 1v6t coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 5-oxo-L-proline + 2 H(2)O = ADP + phosphate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-oxoprolinase subunit A Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58714 (Residues: 1-255; Coverage: 100%)
Gene names: PH0986, pxpA
Sequence domains: LamB/YcsF family
Structure domains: Glycoside hydrolase/deacetylase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I222
Unit cell:
a: 65.366Å b: 74.288Å c: 105.559Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.227
Expression system: Escherichia coli

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